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Mol Cell Biol. 2018 Nov 28;38(24). pii: e00296-18. doi: 10.1128/MCB.00296-18. Print 2018 Dec 15.

Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast.

Author information

1
Department of Biology, Indiana University, Bloomington, Indiana, USA.
2
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
3
UMR7104, Centre National de la Recherche Scientifique, Illkirch, France.
4
U964, Institut National de la Santé et de la Recherche Médicale, Illkirch, France.
5
Université de Strasbourg, Illkirch, France.
6
Department of Biology, Indiana University, Bloomington, Indiana, USA gzentner@indiana.edu.
7
Indiana University Melvin and Bren Simon Cancer Center, Indianapolis, Indiana, USA.

Abstract

Eukaryotic RNA polymerase II (RNAPII) transcribes mRNA genes and non-protein-coding RNA (ncRNA) genes, including those encoding small nuclear and nucleolar RNAs (sn/snoRNAs). In metazoans, RNAPII transcription of sn/snoRNAs is facilitated by a number of specialized complexes, but no such complexes have been discovered in yeast. It has been proposed that yeast sn/snoRNA and mRNA expression relies on a set of common factors, but the extent to which regulators of mRNA genes function at yeast sn/snoRNA genes is unclear. Here, we investigated a potential role for the Mediator complex, essential for mRNA gene transcription, in sn/snoRNA gene transcription. We found that Mediator maps to sn/snoRNA gene regulatory regions and that rapid depletion of the essential structural subunit Med14 strongly reduces RNAPII and TFIIB occupancy as well as nascent transcription of sn/snoRNA genes. Deletion of Med3 and Med15, subunits of the activator-interacting Mediator tail module, does not affect Mediator recruitment to or RNAPII and TFIIB occupancy of sn/snoRNA genes. Our analyses suggest that Mediator promotes PIC formation and transcription at sn/snoRNA genes, expanding the role of this critical regulator beyond its known functions in mRNA gene transcription and demonstrating further mechanistic similarity between the transcription of mRNA and sn/snoRNA genes.

KEYWORDS:

Mediator; RNA polymerase II; snRNA; snoRNA

PMID:
30275344
PMCID:
PMC6275182
[Available on 2019-05-28]
DOI:
10.1128/MCB.00296-18

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