Format

Send to

Choose Destination
J Phys Chem Lett. 2018 Oct 18;9(20):6112-6118. doi: 10.1021/acs.jpclett.8b02686. Epub 2018 Oct 8.

Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells.

Author information

1
Biomedical Research Institute (BIOMED) , Hasselt University , Agoralaan Bldg. C , 3590 Diepenbeek , Belgium.
2
Univ. Savoie Mont Blanc, SYMME , F-74000 Annecy , France.
3
Department of Biosystems Science and Engineering , ETH Zürich , Mattenstrasse 26 , 4058 Basel , Switzerland.
4
Department of Bioengineering , Imperial College London , South Kensington Campus , London SW7 2AZ , U.K.
5
Department of Applied Physics , Université de Genève , Chemin de Pinchat 22 , 1211 Geneva , Switzerland.
6
Laboratory for Enteric Neuroscience (LENS), TARGID , University of Leuven , Herestraat 49 , 3000 Leuven , Belgium.

Abstract

The absence of photobleaching, blinking, and saturation combined with a high contrast provides unique advantages of higher-harmonic generating nanoparticles over fluorescent probes, allowing for prolonged correlation spectroscopy studies. We apply the coherent intensity fluctuation model to study the mobility of second harmonic generating nanoparticles. A concise protocol is presented for quantifying the diffusion coefficient from a single spectroscopy measurement without the need for separate point-spread-function calibrations. The technique's applicability is illustrated on nominally 56 nm LiNbO3 nanoparticles. We perform label-free raster image correlation spectroscopy imaging in aqueous suspension and spatiotemporal image correlation spectroscopy in A549 human lung carcinoma cells. In good agreement with the expected theoretical result, the measured diffusion coefficient in water at room temperature is (7.5 ± 0.3) μm2/s. The diffusion coefficient in the cells is more than 103 times lower and heterogeneous, with an average of (3.7 ± 1.5) × 10-3 μm2/s.

PMID:
30273489
DOI:
10.1021/acs.jpclett.8b02686
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center