Format

Send to

Choose Destination
Nat Biotechnol. 2018 Oct 1. doi: 10.1038/nbt.4261. [Epub ahead of print]

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A.

Zong Y1,2, Song Q1,2, Li C1,2, Jin S1,2, Zhang D1,2, Wang Y1, Qiu JL3, Gao C1,2.

Author information

1
State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
2
University of Chinese Academy of Sciences, Beijing, China.
3
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Abstract

Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context.

PMID:
30272679
DOI:
10.1038/nbt.4261

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center