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Commun Biol. 2018 Sep 12;1:144. doi: 10.1038/s42003-018-0147-y. eCollection 2018.

Cellular-scale probes enable stable chronic subsecond monitoring of dopamine neurochemicals in a rodent model.

Author information

1
1McGovern Institute for Brain Research and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
2
2Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
3
3Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
4
4Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.

Abstract

Chemical signaling underlies both temporally phasic and extended activity in the brain. Phasic activity can be monitored by implanted sensors, but chronic recording of such chemical signals has been difficult because the capacity to measure them degrades over time. This degradation has been attributed to tissue damage progressively produced by the sensors and failure of the sensors themselves. We report methods that surmount these problems through the development of sensors having diameters as small as individual neuronal cell bodies (<10 µm). These micro-invasive probes (µIPs) markedly reduced expression of detectable markers of inflammation and tissue damage in a rodent test model. The chronically implanted µIPs provided stable operation in monitoring sub-second fluctuations in stimulation-evoked dopamine in anesthetized rats for over a year. These findings demonstrate that monitoring of chemical activity patterns in the brain over at least year-long periods, long a goal of both basic and clinical neuroscience, is achievable.

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