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Arch Physiol Biochem. 2018 Sep 29:1-5. doi: 10.1080/13813455.2018.1508237. [Epub ahead of print]

Endothelium-dependent vasodilatory effect of Smilax china Linn. water extract via PI3K/Akt signaling.

Author information

1
a Department of Biomedical Sciences , University of Health and Allied Sciences , Ho , Ghana.
2
b Department of Physiology College of Korean Medicine , Dongguk University , Gyeongju , South Korea.
3
c Korea Promotion Institute for Traditional Medicine Industry , Gyeongsan , South Korea.

Abstract

OBJECTIVE:

The objective of this study was to investigate the pharmacological effect of Smilax china Linn. water extract (SCLWE) on vascular relaxation and its underlying biochemical mechanisms.

METHODOLOGY:

Isolated rat aortic rings were pre-constricted with phenylephrine (PE). This was followed by the cumulative addition of SCLWE. The effect of endothelial nitric oxide and PI3K/Akt on the SCLWE-induced vasodilation was investigated by the pretreatment of endothelium-intact aortic strips with or without NG-nitro-L-arginine methyl ester (L-NAME) or wortmanin before constriction with PE.

RESULTS:

Treatment of PE (1 μM)-pre-contracted aortic strips with SCLWE induced endothelium-dependent relaxation, which was attenuated by L-NAME and wortmanin. Further studies using HUVECs indicated that nitrite production, eNOS and PI3K/PKB (Akt) phosphorylations were increased after exposure to SCLWE but was attenuated by pretreatment with wortmanin.

CONCLUSION:

These results suggest that SCLWE induces vasodilation by augmenting NO production in endothelial cells via PI3K/Akt-dependent eNOS phosphorylation.

KEYWORDS:

L-NAME; Vasodilation; aorta; endothelium; phosphorylation

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