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J Agric Food Chem. 2018 Oct 17;66(41):10760-10768. doi: 10.1021/acs.jafc.8b04676. Epub 2018 Oct 8.

Protective Effect of Bovine Elastin Peptides against Photoaging in Mice and Identification of Novel Antiphotoaging Peptides.

Liu Y1,2, Su G1,2, Zhou F1,2, Zhang J1,2, Zheng L1, Zhao M1,2,3.

Author information

1
School of Food Science and Engineering , South China University of Technology , Guangzhou 510640 , China.
2
Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center , Guangzhou 510650 , China.
3
Beijing Advanced Innovation Center for Food Nutrition and Human Health , Beijing Technology & Business University , Beijing 100048 , China.

Abstract

This study aimed to investigate the protective effects of bovine elastin hydrolysates on UV-induced skin photoaging in mice and to identify the potent antiphotoaging peptides. Results showed that the ingestion of elastin peptides could obviously ameliorate epidermis hyperplasia and fibroblast apoptosis, and increase the content of hydroxyproline and water in photoaging skin in vivo ( p < 0.05). Furthermore, four peptides with elastase inhibitory activity were purified and identified, including GLPY, PY, GLGPGVG, and GPGGVGAL. Interestingly, GLPY and GPGGVGAL exhibited the highest inhibition activity with 58.77% and 42.91% at 10 mΜ, respectively. This might be attributed to the N-terminal Gly, C-terminal Leu, and Pro at the third position of the N-terminus, which showed stronger affinity and interaction with elastase. Moreover, GLPY and GPGGVGAL could also inhibit the apoptosis of fibroblasts effectively at 50 μΜ ( p < 0.01). It suggested that elastin peptides had great potential to prevent and regulate skin photoaging.

KEYWORDS:

antiphotoaging; elastase inhibitory activity; elastin hydrolysates; fibroblast; molecular docking

PMID:
30269487
DOI:
10.1021/acs.jafc.8b04676

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