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Forensic Sci Int. 2018 Nov;292:39-44. doi: 10.1016/j.forsciint.2018.09.009. Epub 2018 Sep 19.

Sensitive determination of nine anticoagulant rodenticides in blood by high resolution mass spectrometry with supported liquid extraction pretreatment.

Author information

1
Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology and Business University, Chongqing 400067, China.
2
Department of Traditional Chinese Medicine, Weinan Central Hospital, Weinan 71400, China.
3
Anhui Supervision Institute of Veterinary Drug and Feed, Hefei, Anhui 230091 China.
4
Chonqing Institute of Forensic Sciences, Chongqing, 400021 China.
5
Key Laboratory of Polar Materials and Devices, Ministry of Education, Department of Electronic Engineering, East China Normal University, Shanghai 200241, China.
6
Chonqing Institute of Forensic Sciences, Chongqing, 400021 China. Electronic address: hao.guo1982@hotmail.com.
7
Key Laboratory of Polar Materials and Devices, Ministry of Education, Department of Electronic Engineering, East China Normal University, Shanghai 200241, China. Electronic address: qbzhao@ee.ecnu.edu.cn.

Abstract

Anticoagulant rodenticides (ARs) have been widely used for controlling rodents in agriculture and households. It often occurs that non-target animals are poisoned by ARs. Also, the abuse of ARs has been often encountered in poisoning and suicide cases. Herein we report the determination of nine commonly used ARs by high resolution mass spectrometry (HRMS) with supported liquid extraction (SLE) pretreatment. The factors affecting SLE (elution solvents and pH values) were systematically tested and optimized. The application of parallel reaction monitoring (PRM) mode led to the highest sensitivity obtained for these compounds, with LODs ranging for 0.006-0.02ng/mL. Reasonable extraction recoveries for all the analytes were obtained ranging in 73.9%-110.7%. Good precision was achieved for the spiked blood samples, with intra-day RSD ranging in 5.0%-9.2% and inter-day RSD ranging in 6.3%-10.5%. The values of ME ranged in 82.9%-103.2% for QC sample, which are reasonable. The application of HRMS in PRM mode also resulted in high selectivity. The method was applied to the detection and quantification of ARs in blood samples from real forensic cases. This methodology possesses high potential for determination of rodenticides in clinical and forensic cases.

KEYWORDS:

Anticoagulant rodenticides; High resolution mass spectrometry; Parallel reaction monitoring; Supported liquid extraction; UHPLC

PMID:
30269045
DOI:
10.1016/j.forsciint.2018.09.009
[Indexed for MEDLINE]

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