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Regul Toxicol Pharmacol. 2018 Nov;99:238-243. doi: 10.1016/j.yrtph.2018.09.029. Epub 2018 Sep 27.

In vitro and in vivo evaluation of the genotoxicity of Eriobotrya japonica leaf extract.

Author information

1
Health Care Institute, Korea Testing and Research Institute, Hwasun, Jeonnam, 58141, Republic of Korea.
2
Health Care Institute, Korea Testing and Research Institute, Hwasun, Jeonnam, 58141, Republic of Korea; College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University, Gwangju, 61186, Republic of Korea.
3
Korea INS Pharm Research Institute, Hwasun, Jeonnam, 58143, Republic of Korea.
4
College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University, Gwangju, 61186, Republic of Korea.
5
College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University, Gwangju, 61186, Republic of Korea. Electronic address: toxkim@jnu.ac.kr.

Abstract

Eriobotrya japonica leaf is included in the Chinese Pharmacopoeia, and is widely used as a medicinal material in traditional medicine. The present study investigated the potential genotoxic effects of E. japonica leaf extract (EJE) using three standard battery systems. Genotoxicity tests were conducted following the test guidelines of the Organisation for Economic Cooperation and Development (OECD) and Ministry of Food and Drug Safety (MFDS), with application of Good Laboratory Practice. The bacterial reverse mutation test was conducted using the pre-incubation method in the presence or absence of the metabolic activation system (S9 mixture). The in vitro chromosome aberration test was performed using cultured Chinese hamster lung cell line in the presence or absence of the S9 mixture. The in vivo micronucleus test was performed using ICR mice. The bacterial reverse mutation test with Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537 and Escherichia coli strain WP2uvrA showed that EJE did not induce gene mutations at any dose level in all the strains tested. EJE also did not show any chromosomal aberrations in the in vitro chromosomal aberration test and in the in vivo micronucleus test. These results showed that EJE did not induce mutagenicity or clastogenicity in either in vitro or in vivo systems.

KEYWORDS:

Bacterial reverse mutation test; Chromosome aberration test; Eriobotrya japonica leaf extract; Genotoxicity; Micronucleus test

PMID:
30268829
DOI:
10.1016/j.yrtph.2018.09.029
[Indexed for MEDLINE]

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