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FEBS J. 2018 Sep 29. doi: 10.1111/febs.14666. [Epub ahead of print]

A guide to microRNA-mediated gene silencing.

Author information

1
Oncology division (St-Patrick Research Group in Basic Oncology), CHU de Québec-Université Laval Research Center, Quebec City, Canada.
2
Laval University Cancer Research Centre, Quebec City, Canada.

Abstract

As an important regulator of gene expression in eukaryotes, microRNAs (miRNAs) have been subject to extensive research to understand their pleiotropic roles in development and pathological processes. Here, we review strategies that have been developed to map microRNA-target interactions and the most effective methods to attribute post-transcriptional gene regulation through miRNA-mediated silencing. We further discuss the importance of the biological context for silencing.

KEYWORDS:

gene regulation; miRISC; microRNA target; microRNA target validation

PMID:
30267606
DOI:
10.1111/febs.14666

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