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Chin J Integr Med. 2018 Sep 28. doi: 10.1007/s11655-018-2932-8. [Epub ahead of print]

Purines Change at Acupoints along the Pericardium Meridian in Healthy and Myocardial Ischemic Rats.

Author information

1
Department of Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
2
The Second Clinical Medical College of Nanjing University of Chinese Medicine, Nanjing, 210046, China.
3
Department of Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China. acuresearch@126.com.

Abstract

OBJECTIVE:

To quantify the purine concentrations of the acupoints along the pericardium and nonpericardium meridians under healthy and myocardial ischemia conditions to investigate the relationship between acupoint purine change and body functional status in rats.

METHODS:

A total of 70 rats underwent an operation for myocardial ischemia, while 40 of them survived. They were randomly assigned to the following 5 subgroups: Neiguan (PC 6), Quze (PC 3), Tianquan (PC 2), Quchi (LI 11), and Jianyu (LI 15). Simultaneously, another 40 healthy rats were also randomized into the same 5 subgroups as the control group. The tissue flfl uids at the acupoints were collected by microdialysis for 30 min. Subsequently, the concentration of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and adenosine (ADO) were quantififi ed using the high-performance liquid chromatography method.

RESULTS:

Compared with the healthy group, the ADO at PC 6 (P=0.012), PC 3 (P=0.038), PC 2 (P=0.024), and LI 15 (P=0.042) obviously increased in the model group, while no signififi cant difference was observed at LI 11 (P=0.201). However, ATP, ADP, and AMP manifested no significant changes in these areas, except for ATP at LI 15 (P=0.036).

CONCLUSIONS:

Myocardial ischemia could induce an increase in ADO at acupoints of the upper arm and shoulder area, suggesting that the body functional status could affect the responsiveness of acupoints. The status of these acupoints could be pathogenically activated by disease, and distribution following some specififi c courses.

KEYWORDS:

acupoint; adenosine; highperformance liquid chromatography; microdialysis; myocardial ischemia; pericardium meridian; purine

PMID:
30264268
DOI:
10.1007/s11655-018-2932-8

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