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Food Sci Biotechnol. 2018 Feb 17;27(4):1119-1127. doi: 10.1007/s10068-018-0335-z. eCollection 2018 Aug.

Diversity analysis of Saccharomyces cerevisiae isolated from natural sources by multilocus sequence typing (MLST).

Author information

1
1Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104 Korea.
2
2Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689 Korea.
3
Research Institute, Kooksoondang Brewery Co. Ltd., Seongnam, 13202 Korea.
4
4College of Pharmacy, Sookmyung Women's University, Seoul, 04310 Korea.
5
5Department of Food Science and Technology, Chung-Ang University, Ansung, 17546 Korea.
6
6Brewing Research Center, Academic Industry Cooperation, Hankyong National University, Anseong, 17579 Korea.

Abstract

We used multilocus sequence typing (MLST) to analyze the diversity of natural isolates of Saccharomyces cerevisiae, the most important microorganism in alcoholic fermentation. Six loci, ADP1, RPN2, GLN4, ACC1, MET4, and NUP116, in S. cerevisiae genome were selected as MLST markers. To investigate genetic diversity within S. cerevisiae, 42 S. cerevisiae isolated from natural sources in Korea as well as six S. cerevisiae obtained from Genbank and four industrial S. cerevisiae were examined using MLST. Twenty-six polymorphic sites were found in the six loci. Among them, ACC1 had the most genetic variation with eight polymorphic sites. MLST differentiated the 52 strains into three clades. Alcohol fermentation results revealed that S. cerevisiae in Clade III produced less alcohol than those in Clades I and II. These results suggested that MLST is a powerful tool to differentiate S. cerevisiae and can potentially be used to select S. cerevisiae suitable for industrial use.

KEYWORDS:

Housekeeping genes; MLST; Makgeolli; Saccharomyces cerevisiae

PMID:
30263842
PMCID:
PMC6085237
[Available on 2019-02-17]
DOI:
10.1007/s10068-018-0335-z

Conflict of interest statement

Compliance with ethical standardsThe authors declare that they have no conflict of interest.

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