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Food Sci Biotechnol. 2017 Dec 12;27(2):451-459. doi: 10.1007/s10068-017-0253-5. eCollection 2018 Apr.

Application of stabilizer improves stability of nanosuspended branched-chain amino acids and anti-inflammatory effect in LPS-induced RAW 264.7 cells.

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1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029 Korea.
R&D Center, Daesang Corporation, Icheon, Gyoenggi-do 17384 Korea.
3Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 01811 Korea.
4Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826 Korea.


This study investigated the use of polyglyceryl esters (PGE) as stabilizer in improving stability and anti-inflammatory activity of nanosuspended branched-chain amino acids (BCAAs). BCAAs nanosuspended with stabilizer (BS) exhibited improved stability at concentration of 5% saturation level during storage as compared to BCAAs nanosuspended with aqueous solution (BA). Additionally, anti-inflammatory activity of BS was found to be greater than that of BA. Nitric oxide scavenging activity was found to be dose-dependent, with activity of BS in sodium nitroprusside system being significantly higher than that of BA (p < 0.05) at 2.5-20 mg/mL. BS also possesses greater inhibitory activity on production of pro-inflammatory factors including inducible nitric oxide synthase, cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells through suppressed phosphorylation of p65 subunit of NF-κB at 0.5, 2, 8 mg/mL. These results suggest that PGE used as stabilizer improves solubility and biological activity of nanosuspended BCAAs.


Anti-inflammatory; Branched-chain amino acids; High pressure homogenization; RAW 264.7 macrophages; Stabilizer

Conflict of interest statement

Compliance with ethical standardsThe authors have declared no conflict of interest.

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