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Food Sci Biotechnol. 2017 Dec 20;26(6):1715-1723. doi: 10.1007/s10068-017-0230-z. eCollection 2017.

The suppressive effect of Gelidium amansi-EtOH extracts on the adipogenesis with MAPK signals in adipocytes with or without macrophages.

Author information

1
1Research Institute of Obesity Sciences, Sungshin Women's University, Seoul, 01133 Republic of Korea.
2
Seojin Biotech Co. Ltd., Dongbaekjunang-ro, Giheung-Gu, Yongin-si, Gyeonggi-do 17015 Republic of Korea.
3
3Department of Food and Nutrition and Research Institute of Obesity Science, Sungshin Women's University, Seoul, 01133 Republic of Korea.

Abstract

To elucidate the anti-inflammatory and anti-adipogenetic effects of Gelidium amansii (GA) ethanol extracts and their mechanisms, we performed two culture systems, adipocytes cultured with or without macrophages. Purified GA-3 fraction (GAE) contains high flavonoids and phenolics, reduced the mRNA levels of PPARγ and C/EBPα with GLUT4 expression in adipocyte with or without macrophages. GAE also increased the protein expression of HSL and ATGL enzymes, lipolysis biomarkers in fat cells. In co-culture system, GAE suppressed not only the transcription factors for adipogenesis, but also the production of pro-inflammatory cytokines, TNF-α. Compared to MAPK pathways such as JNK and p-38, the phosphorylation of both ERK1/2 (Thr202/Tyr204) was strongly suppressed by GAE with dose-dependent manner in both culture system. Otherwise, an increased JNK expression caused by GAE treatments blocked an insulin-induced GLUT4 translocation in adipocytes culture. In conclusion, GAE depressed the expression of adipogenetic genes, corresponding to a reduction in fat accumulation while preadipocytes developed into adipocytes with the modulation of MAPK pathways and inflammatory cytokines.

KEYWORDS:

Adipocytes; ERK1/2; Gelidium amansii; MAPK; Macrophages; TNF-α

Conflict of interest statement

Compliance with ethical standardsThe authors declare no conflict of interest.

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