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Indian J Microbiol. 2018 Dec;58(4):415-422. doi: 10.1007/s12088-018-0737-x. Epub 2018 May 2.

Genomics and Biochemistry of Metabolic Pathways for the C1 Compounds Utilization in Colorless Sulfur Bacterium Beggiatoa leptomitoformis D-402.

Author information

1
1Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, Voronezh, Russia 394018.
2
2All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region Russia 142290.
3
3Federal State Institution "Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences", Moscow, Russia 117312.
4
4Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region Russia 142290.
5
5Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow, Russia 143028.

Abstract

The metabolic pathways of one-carbon compounds utilized by colorless sulfur bacterium Beggiatoa leptomitoformis D-402 were revealed based on comprehensive analysis of its genomic organization, together with physiological, biochemical and molecular biological approaches. Strain D-402 was capable of aerobic methylotrophic growth with methanol as a sole source of carbon and energy and was not capable of methanotrophic growth because of the absence of genes of methane monooxygenases. It was established that methanol can be oxidized to CO2 in three consecutive stages. On the first stage methanol was oxidized to formaldehyde by the two PQQ (pyrroloquinolinequinone)-dependent methanol dehydrogenases (MDH): XoxF and Mdh2. Formaldehyde was further oxidized to formate via the tetrahydromethanopterin (H4MPT) pathway. And on the third stage formate was converted to CO2 by NAD+-dependent formate dehydrogenase Fdh2. Finally, it was established that endogenous CO2, formed as a result of methanol oxidation, was subsequently assimilated for anabolism through the Calvin-Benson-Bassham cycle. The similar way of one-carbon compounds utilization also exists in representatives of another freshwater Beggiatoa species-B. alba.

KEYWORDS:

Beggiatoa leptomitoformis D-402; C1 compounds utilization; Colorless sulfur bacteria; Methylotrophic growth

PMID:
30262951
PMCID:
PMC6141403
[Available on 2019-12-01]
DOI:
10.1007/s12088-018-0737-x

Conflict of interest statement

Compliance with Ethical StandardsThe authors declare that they have no conflict of interest.

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