Format

Send to

Choose Destination
BMC Complement Altern Med. 2018 Sep 27;18(1):263. doi: 10.1186/s12906-018-2320-8.

Inhibitory effects of Euphorbia supina on Propionibacterium acnes-induced skin inflammation in vitro and in vivo.

Author information

1
Department of Oriental Medicine Resources, Chonbuk National University, 79 Gobongro, Iksan, Jeollabuk-do, 54596, South Korea.
2
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicine Research Institute, Wonkwang University, Iksan, Jeollabuk-do, 54538, South Korea.
3
Department of Pharmacy, College of Pharmacy, Dongduk Woman's University, 23-1 Wolgok-Dong, SungBuk-Gu, Seoul, 02748, South Korea.
4
Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588, Japan.
5
Department of Ecology Landscape Architecture-Design, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.
6
Department of Oriental Medicine Resources, Chonbuk National University, 79 Gobongro, Iksan, Jeollabuk-do, 54596, South Korea. jongsik.jin@jbnu.ac.kr.
7
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicine Research Institute, Wonkwang University, Iksan, Jeollabuk-do, 54538, South Korea. ymlee@wku.ac.kr.

Abstract

BACKGROUND:

Euphorbia supina (ES) plant has been used as treatment for inflammatory conditions. The antibacterial effect and the anti-inflammatory mechanism of ES for Propionibacterium (P.) acnes-induced inflammation in THP-1 cells and acne animal model remain unclear. Therefore, the objective of the present study was to determine the antibacterial and anti-inflammatory activities of ES against P. acnes, the etiologic agent of skin inflammation.

METHOD:

The antibacterial activities of ES were tested with disc diffusion and broth dilution methods. Cytotoxicity of ES at different doses was evaluated by the MTT assay. THP-1 cells were stimulated by heat-killed P. acnes in the presence of ES. The pro-inflammatory cytokines and mRNA levels were measured by ELISA and real-time-PCR. MAPK expression was analyzed by Western blot. The living P. acnes was intradermally injected into the ear of BLBC/c mice. Subsequently, chemical composition of ES was analyzed by liquids chromatography-mass spectrometry (LC-MS).

RESULT:

ES had stronger antibacterial activity against P. acnes and inhibitory activity on lipase. ES had no significant cytotoxicity on THP-1 cells. ES suppressed the mRNA levels and production of IL-8, TNF-a, IL-1β in vitro. ES inhibited the expression levels of pro-inflammatory cytokines and the MAPK signaling pathway. Ear thickness and inflammatory cells were markedly reduced by ES treatment. Protocatechuic acid, gallic acid, quercetin, and kaempferol were detected by LC-MS analysis in ES.

CONCLUSIONS:

Our results demonstrate antibacterial and anti-inflammatory activities of ES extract against P. acnes. It is suggested that ES extract might be used to treatment anti-inflammatory skin disease.

PMID:
30261862
PMCID:
PMC6161423
DOI:
10.1186/s12906-018-2320-8
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center