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Int Immunopharmacol. 2018 Nov;64:424-431. doi: 10.1016/j.intimp.2018.09.029. Epub 2018 Sep 25.

Potential therapeutic role of Co-Q10 in alleviating intervertebral disc degeneration and suppressing IL-1β-mediated inflammatory reaction in NP cells.

Author information

1
Department of Orthopaedic Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, PR China.
2
The Third People's Hospital of Jinan, Jinan, Shandong 250101, PR China.
3
Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, PR China.
4
Department of Pathology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, PR China.
5
Department of Orthopaedic Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, PR China. Electronic address: professorzhaoyp@163.com.

Abstract

Coenzyme Q10 (Co-Q10) is extraordinarily popular and has been used in abundant interventions as an antioxidant reagent that participates in numerous oxidation reactions. According to substantial evidence previously reported, interleukin-1β (IL-1β) is deemed to be one of the chief orchestrator molecules in the degeneration of intervertebral disc (IVD). However, it is unknown whether Co-Q10 is able to protect against IVD degeneration. In the current study, mouse-derived IVDs as well as primary human nucleus pulposus (NP) cells were isolated and cultured. NP cells were stimulated with IL-1β, with or without selective addition of Co-Q10 to investigate the therapeutic effect of Co-Q10 on IVD degeneration. Levels of IL-1β-induced inflammatory biomarkers including TNF-α, COX-2, IL-6 and iNOS were reduced by Co-Q10, which was possibly associated with inhibition of NF-κB signaling activation. Furthermore, Co-Q10 maintained the production of anabolic biomarkers in NP cells such as collagen 2, aggrecan and Sox-9 and altered the enhanced catabolism induced by IL-1β. Moreover, the therapeutic role of Co-Q10 in sustaining IVD tissue-enhanced anabolism is potentially dependent on activation of the Akt signaling pathway. In summary, Co-Q10 may potentially represent an available molecular target that may shed light on approaches to the prevention and treatment of IVD degeneration in the future.

KEYWORDS:

Coenzyme Q10; IL-1β; Inflammation; Intervertebral disc degeneration

PMID:
30261465
DOI:
10.1016/j.intimp.2018.09.029
[Indexed for MEDLINE]

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