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J Biomater Sci Polym Ed. 2018 Dec;29(17):2168-2186. doi: 10.1080/09205063.2018.1528520. Epub 2018 Dec 14.

Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.

Li M1,2,3, Wang Y2,4, Zhang J2,4, Cao Z5, Wang S5, Zheng W1,2,3, Li Q1,2,3, Zheng T1,2,3, Wang X6, Xu Q2,4, Chen Z1,2,3.

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a Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education , Beijing , China.
b Center of Neural Injury and Repair, Beijing Institute for Brain Disorders , Beijing , China.
c Center of Parkinson's Disease, Beijing Institute for Brain Disorders , Beijing , China.
d Department of Neurobiology , Capital Medical University , Beijing , China.
e School of Materials Science and Engineering, Tsinghua University , Beijing , China.
f Institute for Regenerative Medicine and Biomimetic Materials, Department of Materials Science and Engineering , Tsinghua University , Beijing , China.


A combinatory approach using biomaterials together with cells may improve the efficacy of cell therapy for treatment of various diseases/indications. In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type. Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only. Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction. However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture. NSCs and derivatives hold promise in treating neurological diseases. On aligned fibrin, NSCs could differentiate and grow neurites in a directional manner before fibrin was degraded in 2 days. On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way. Fibroblast graft are useful in many indications, such as in skin burns. Fibroblasts generally grew better on the tested materials than did the neural cells, and fibroblasts could grow directionally on the aligned fibrin and scattered around on the alveolate collagen. The study provided information which may be used to further optimize the materials to support culture of each type of cells.


aligned biomaterials; fibroblasts; neural stem cells; pyramidal neurons; tissue repair

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