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Mol Cell Proteomics. 2018 Sep 26. pii: mcp.RA118.000808. doi: 10.1074/mcp.RA118.000808. [Epub ahead of print]

microRNA-222 Attenuates Mitochondrial Dysfunction During Transmissible Gastroenteritis Virus Infection.

Author information

1
Northwest A&F University, China.
2
Huyi District Center for Animal Disease Control and Prevention, China.
3
College of Veterinary Medicine, Northwest A&F University, China dwtong@nwsuaf.edu.cn.

Abstract

Transmissible gastroenteritis virus (TGEV) is a member of Coronaviridae family. Our previous research showed that TGEV infection could induce mitochondrial dysfunction and up-regulate miR-222 level. Therefore, we presumed that miR-222 might be implicated in regulating mitochondrial dysfunction induced by TGEV infection. To verify the hypothesis, the effect of miR-222 on mitochondrial dysfunction was tested and we showed that miR-222 attenuated TGEV-induced mitochondrial dysfunction. To investigate the underlying molecular mechanism of miR-222 in TGEV-induced mitochondrial dysfunction, a quantitative proteomic analysis of PK-15 cells that were transfected with miR-222 mimics and infected with TGEV was performed. In total, 4151 proteins were quantified and 100 differentially expressed proteins were obtained (57 up-regulated, 43 down-regulated), among which thrombospondin-1 (THBS1) and cluster of differentiation 47 (CD47) were down-regulated. THBS1 was identified as the target of miR-222. Knockdown of THBS1 and CD47 decreased mitochondrial Ca2+ level and increased mitochondrial membrane potential (MMP) level. Reversely, overexpression of THBS1 and CD47 elevated mitochondrial Ca2+ level and reduced mitochondrial membrane potential (MMP) level. Together, our data establish a significant role of miR-222 in regulating mitochondrial dysfunction in response to TGEV infection.

KEYWORDS:

HPLC; Mitochondria function or biology; Molecular biology*; Pathway Analysis; Tandem Mass Spectrometry; Viruses; cluster of differentiation 47 (CD47); microRNA-222; proteomics; thrombospondin-1 (THBS1); transmissible gastroenteritis virus

PMID:
30257878
DOI:
10.1074/mcp.RA118.000808
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