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J Biomed Mater Res B Appl Biomater. 2018 Sep 25. doi: 10.1002/jbm.b.34171. [Epub ahead of print]

Bone regeneration in rabbit calvarial critical-sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture.

Author information

1
Department of Cranio-Maxillofacial Surgery and GROW School for Oncology and Developmental Biology, Maastricht University Medical Center, Maastricht, The Netherlands.
2
Department of Oral and Maxillofacial Surgery, RWTH Aachen University, Aachen, Germany.
3
Department of Surgical Sciences, Faculty of Dentistry, Health Sciences Center, Kuwait University, Safat, Kuwait.
4
Department of Experimental Molecular Imaging, RWTH Aachen University, Aachen, Germany.
5
Animal Resources Centre, Health Sciences Center, Kuwait University, Safat, Kuwait.

Abstract

The purpose of this study was to evaluate bone healing in calvarial defects using two bone graft substitute materials; biphasic beta-tricalcium phosphate/hydroxyapatite in hydrogel (ß-TCP/HA) versus composite non-demineralized xenogenic dentin with ß-TCP/HA mixture. Full thickness critical-sized defects were created bilaterally in 10 New Zealand male rabbits. Seven defects were left empty, six filled with biphasic tricalcium phosphate putty, and seven were filled with composite non-demineralized xenogenic dentin with biphasic tricalcium phosphate. Animals were sacrificed at eight weeks postoperatively and the healing of the biomaterial-filled defects was compared radiographically and by histomorphometry. Micro-computed tomography (μCT) was utilized to analyze the osteogenesis and healing patterns of the defects. Quantitative analysis of volume fraction (%) of the newly formed bone and remaining graft material (FV=filling volume/TV=tissue volume) and mean intensity [HU] in the defects were evaluated. Defects filled with composite dentin with biphasic tri-calcium phosphate showed volume fraction (FV/TV) in the order of 55.81% ± 17.72%, whereas defects filled with only biphasic tricalcium phosphate showed a fraction of 39.84% ± 16.06%, which represent the ratio of remaining graft material and new bone formation to the tissue volume. The empty negative control defects showed a volume fraction of 19.14% ± 8.787%. Histological analysis showed significant percentage increase in bone formation and residual graft with the composite Dentin/ß-TCP group after 8 weeks. The findings suggest that composite xenogenic dentin with biphasic tricalcium phosphate showed improved osteogenesis when compared to biphasic tricalcium phosphate without the addition of non-demineralized dentin.

KEYWORDS:

X-ray micro-computed tomography; bone regeneration; bone substitutes; bone transplantation; dentin; skull

PMID:
30253039
DOI:
10.1002/jbm.b.34171

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