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New Phytol. 2018 Sep 25. doi: 10.1111/nph.15439. [Epub ahead of print]

EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis.

Author information

1
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Seeland OT Gatersleben, Germany.
2
Department of Bioinformatics, Martin-Luther-University, 06120, Halle, Germany.
3
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076, Tübingen, Germany.
4
Gregor Mendel Institute of Molecular Plant Biology, 1030, Vienna, Austria.

Abstract

Plant-specific EFFECTORS OF TRANSCRIPTION (ET) are characterised by a variable number of highly conserved ET repeats, which are involved in zinc and DNA binding. In addition, ETs share a GIY-YIG domain, involved in DNA nicking activity. It was hypothesised that ETs might act as epigenetic regulators. Here, methylome, transcriptome and phenotypic analyses were performed to investigate the role of ET factors and their involvement in DNA methylation in Arabidopsis thaliana. Comparative DNA methylation and transcriptome analyses in flowers and seedlings of et mutants revealed ET-specific differentially expressed genes and mostly independently characteristic, ET-specific differentially methylated regions. Loss of ET function results in pleiotropic developmental defects. The accumulation of cyclobutane pyrimidine dimers after ultraviolet stress in et mutants suggests an ET function in DNA repair.

KEYWORDS:

Arabidopsis thaliana ; DNA methylation; DNA repair; EFFECTOR OF TRANSCRIPTION (ET); methylome

PMID:
30252137
DOI:
10.1111/nph.15439

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