Carrageenan stimulates reduction of nitroblue tetrazolium by human neutrophils without membrane depolarization, myeloperoxidase secretion, or increased oxygen consumption

Inflammation. 1986 Dec;10(4):425-34. doi: 10.1007/BF00915826.

Abstract

Carrageenan, a sulfated polyanionic polysaccharide, is commonly used to induce inflammation in experimental animals, and this model is used to screen for the effectiveness of antiinflammatory drugs. Carrageenan-induced inflammation has been attributed to a variety of autocoids including histamine, bradykinin, complement, superoxide, and prostaglandins. This study examines the effects of carrageenan on human PMN in a serum-free system. Carrageenan was found to stimulate the reduction of NBT by PMNs without stimulating membrane depolarization, oxygen consumption, H2O2 production, or myeloperoxidase secretion. Carrageenan stimulates a heat-labile, NBT-reducing system which is unassociated with the usual stimulus-response coupling seen with other PMN activators such as PMA, FMLP, and zymosan.

MeSH terms

  • Carrageenan / pharmacology*
  • Cell Membrane / physiology
  • Humans
  • Hydrogen Peroxide / blood
  • In Vitro Techniques
  • Kinetics
  • Membrane Potentials / drug effects
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Nitroblue Tetrazolium / metabolism*
  • Oxidation-Reduction
  • Oxygen Consumption / drug effects*
  • Peroxidase / blood*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tetrazolium Salts / metabolism*

Substances

  • Tetrazolium Salts
  • Nitroblue Tetrazolium
  • Carrageenan
  • Hydrogen Peroxide
  • Peroxidase
  • Tetradecanoylphorbol Acetate