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J Craniomaxillofac Surg. 2018 Nov;46(11):1899-1904. doi: 10.1016/j.jcms.2018.08.008. Epub 2018 Aug 29.

Aberrant expression of the innate restriction factor bone marrow stromal antigen-2 in primary Sjögren's syndrome.

Author information

1
Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Zhizaoju Road 639, Shanghai 200011, China; National Clinical Research Center for Oral Diseases, Zhizaoju Road 639, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Zhizaoju Road 639, Shanghai 200011, China. Electronic address: cctdcq@126.com.
2
Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Zhizaoju Road 639, Shanghai 200011, China; National Clinical Research Center for Oral Diseases, Zhizaoju Road 639, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Zhizaoju Road 639, Shanghai 200011, China. Electronic address: shihuan1312@163.com.
3
Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Zhizaoju Road 639, Shanghai 200011, China; National Clinical Research Center for Oral Diseases, Zhizaoju Road 639, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Zhizaoju Road 639, Shanghai 200011, China.
4
Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Zhizaoju Road 639, Shanghai 200011, China; National Clinical Research Center for Oral Diseases, Zhizaoju Road 639, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Zhizaoju Road 639, Shanghai 200011, China. Electronic address: ycq616@hotmail.com.
5
Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Zhizaoju Road 639, Shanghai 200011, China; National Clinical Research Center for Oral Diseases, Zhizaoju Road 639, Shanghai 200011, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Zhizaoju Road 639, Shanghai 200011, China. Electronic address: zhenglingyan73@163.com.

Abstract

OBJECTIVE:

The objective of this study was to analyze bone marrow stromal antigen-2 (BST-2) levels in labial glands, total peripheral blood mononuclear cells (PBMCs) and PBMC subpopulations from primary Sjögren's syndrome (pSS) patients and determine the correlation between BST-2 expression and clinical characteristics.

MATERIALS AND METHODS:

PBMC subsets were positively separated using magnetic microbeads. BST-2 mRNA levels in labial glands, total PBMCs and PBMC subsets of 30 pSS and 30 healthy control (HC) subjects were investigated using real-time polymerase chain reaction (RT-PCR). Distribution of BST-2-positive cells in the labial glands was assessed by immunohistochemistry.

RESULTS:

BST-2 was significantly increased in pSS labial glands and was positively correlated with the VAS value for parotid gland swelling and rheumatoid factor and β2-microglobulin serum levels. BST-2 levels were statistically different between pSS patients with positive and negative expression of anti-SSA antibody. Positive focal infiltrating lymphocytes and adjacent ductal epithelial cells were observed in labial glands from pSS patients, while there were a few scattered positive ductal epithelial cells in controls. BST-2 was also up-regulated in CD19+ B cells and the remaining CD4-CD8-CD19- PBMCs.

CONCLUSION:

BST-2 was aberrantly expressed in pSS patients, and expression in labial glands was positively correlated with important clinical characteristics; thus, it may be a potential biomarker of pSS activity.

KEYWORDS:

BST-2; CD19; Labial glands; PBMC; Primary Sjögren's syndrome

PMID:
30249485
DOI:
10.1016/j.jcms.2018.08.008
[Indexed for MEDLINE]

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