Format

Send to

Choose Destination
Nutrients. 2018 Sep 22;10(10). pii: E1356. doi: 10.3390/nu10101356.

A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate.

Author information

1
Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo 173-8602, Japan. shigemura@tokyo-kasei.ac.jp.
2
Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo 173-8602, Japan. k151907@tokyo-kasei.ac.jp.
3
Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo 173-8602, Japan. tateno-m@tokyo-kasei.ac.jp.
4
Q'sai Co., Ltd., 1-7-16 Kusagae, Chuo-ku Fukuoka City 810-8606, Japan. a_suzuki@kyusai.co.jp.
5
Q'sai Co., Ltd., 1-7-16 Kusagae, Chuo-ku Fukuoka City 810-8606, Japan. kurokawa@kyusai.co.jp.
6
Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo 173-8602, Japan. satouy@tokyo-kasei.ac.jp.
7
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, Kyoto 606-8502, Japan. kensato@kais.kyoto-u.ac.jp.

Abstract

Levels of short linear hydroxyproline (Hyp)-containing peptides, such as prolyl-hydroxyproline (Pro-Hyp), increase in human blood after the ingestion of collagen hydrolysate, which has been associated with beneficial effects for human skin and joints. The present study demonstrates the presence of a novel food-derived collagen peptide, cyclic Pro-Hyp, in human blood after the ingestion of collagen hydrolysate. The cyclic Pro-Hyp levels in plasma samples were estimated by liquid chromatography mass spectrometry (LC-MS). Cyclic Pro-Hyp levels significantly increased in the plasma after ingestion of collagen hydrolysate, reaching a maximum level after 2 h and then decreasing. The maximum level of cyclic Pro-Hyp in plasma ranged from 0.1413 to 0.3443 nmol/mL, representing approximately 5% of linear Pro-Hyp in plasma after ingestion of collagen hydrolysate. Addition of cyclic Pro-Hyp in medium at 7 nmol/mL significantly enhanced the growth rate of mouse skin fibroblasts on collagen gel more extensively compared to linear Pro-Hyp.

KEYWORDS:

Pro-Hyp; collagen hydrolysate; collagen peptides; cyclic Pro-Hy; fibroblasts; mouse skin

PMID:
30248982
PMCID:
PMC6213548
DOI:
10.3390/nu10101356
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center