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J Vis Exp. 2018 Sep 7;(139). doi: 10.3791/57981.

Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury.

Author information

1
Department of Neurological Surgery, Ohio State University.
2
Department of Surgery, Ohio State University.
3
Department of Surgery, Duke University.
4
Department of Internal Medicine, Ohio State University.
5
Department of Neurological Surgery, Ohio State University; shahid.nimjee@osumc.edu.

Abstract

Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.

PMID:
30247470
PMCID:
PMC6235140
[Available on 2020-09-07]
DOI:
10.3791/57981
[Indexed for MEDLINE]

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