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Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author information

1
Max Planck Institute for Marine Microbiology, Bremen, Germany.
2
Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
3
University of Chinese Academy of Sciences, Beijing, China.
4
College of Ocean, Hebei Agricultural University, Qinhuangdao, China.
5
MARUM, Center for Marine Environmental Sciences, University of Bremen, Bremen, Germany.
6
Pharmaceutical Biotechnology, Institute of Pharmacy, University Greifswald, Greifswald, Germany.
7
Institute of Marine Biotechnology, Greifswald, Germany.
8
Institute of Microbiology, University Greifswald, Greifswald, Germany.

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest α- and β-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for α- and β-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.

PMID:
30246424
DOI:
10.1111/1462-2920.14414

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