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Behav Brain Res. 2018 Sep 19. pii: S0166-4328(18)31068-4. doi: 10.1016/j.bbr.2018.09.012. [Epub ahead of print]

Behavioral response to fiber feedingis cohort-dependent and associated with gut microbiota composition in mice.

Author information

1
Division of Nutritional Sciences, University of Illinois Urbana-Champaign, IL, United States.
2
Center for Microbial Pathogenesis, Nationwide Children's Hospital, Ohio State University, Columbus, OH, United States.
3
School of Health Studies, University of Memphis, Memphis, TN, United States.
4
Division of Nutritional Sciences, University of Illinois Urbana-Champaign, IL, United States; Department of Animal Sciences, University of Illinois Urbana-Champaign, IL, United States.
5
Department of Kinesiology and Community Health, University of Illinois Urbana-Champaign, IL, United States.
6
Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, IL, United States.
7
Department of Animal Sciences, University of Illinois Urbana-Champaign, IL, United States.
8
Division of Nutritional Sciences, University of Illinois Urbana-Champaign, IL, United States; Department of Animal Sciences, University of Illinois Urbana-Champaign, IL, United States; Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, IL, United States.
9
Division of Nutritional Sciences, University of Illinois Urbana-Champaign, IL, United States; Department of Kinesiology and Community Health, University of Illinois Urbana-Champaign, IL, United States. Electronic address: Woods1@illinois.edu.

Abstract

Recent data has supported a role for the gut microbiota in improving cognition and shaping behavior. Here, we assessed whether pectin, a soluble, fermentable fiber, could enhance learning and memory in mice. Two cohorts of young male C57Bl/6 J mice, C1 (n = 20) and C2 (n = 20), were obtained from Jackson Laboratory and randomized to semi-purified AIN-93 M diets containing 5% pectin (n = 10) or cellulose (n = 10). After 16 weeks, learning and memory was assessed by Morris Water Maze (MWM) and microbiota composition was analyzed by 16S rRNA sequencing. Despite identical treatment, we observed differences in learning and memory abilities between cohorts, along with distinct microbiotas. In C1, pectin-fed mice spent a higher percentage of time in the target quadrant at the 24-h probe trial of the MWM versus cellulose-fed mice; in C2, no effect of pectin was observed. In both cohorts, UniFrac distance revealed significant differences in gut microbial communities between cellulose-fed and pectin-fed mice. UniFrac analysis also revealed significantly different bacterial communities between cohorts. Further analysis demonstrated that the microbial genera Oscillospira, Bilophila, and Peptostreptococcoceae were more abundant in C1 versus C2, and positively associated with distance from the platform during the 24-h probe test. These data support previous findings that differences in the gut microbiota may play a role in host response to a dietary intervention and could partly explain irreproducibility in psychological and behavioral experiments. Further research is needed to determine if a causal relationship exists.

KEYWORDS:

Cohort; Fiber; Gut microbiota; Learning; Memory; Reproducibility

PMID:
30243767
DOI:
10.1016/j.bbr.2018.09.012

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