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Carbohydr Polym. 2018 Dec 1;201:500-513. doi: 10.1016/j.carbpol.2018.08.106. Epub 2018 Aug 27.

Structural elucidation and immune-enhancing activity of peculiar polysaccharides fractioned from marine clam Meretrix meretrix (Linnaeus).

Author information

1
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China. Electronic address: 993wlc@njucm.edu.cn.
2
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Pharmacy Department, Kunshan TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, Jiangsu 215330, China.
3
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
4
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China. Electronic address: whao5795@vip.sina.cn.

Abstract

The marine clam Meretrix meretrix was subjected to extraction with a combined procedure of water decoction, ethanol precipitation, and deproteinization to yield a water-soluble polysaccharide coded as MMP. The product was fractionated with DEAE-52 cellulose and resulted in two soluble fractions designated as MMP-2 and -3. The structures of the fractions were determined by chemical and physicochemical analysis, and their immunomodulation activities and cytokine production were assayed by lymphopoiesis. Results showed that MMP-2 and -3 were glucomannan and glucogalactan homologs, respectively. They possess average molecular masses of 272 and 257 kDa, respectively, and were elucidated as branched copolymers, elaborated as α(1→4)-glucan branching with mannose or galactose via β(1→6)-linkage. Bioassay indicated that MMP-2 and -3 held moderate potentials in restoring immune function because they augment splenocyte proliferation and enhance the cytokine secretion of lymphocytes dose-dependently. We speculated that their bioactivities are closely correlated with their peculiar structures.

KEYWORDS:

Immune activity; M. meretrix; Polysaccharide; Structure elucidation

PMID:
30241847
DOI:
10.1016/j.carbpol.2018.08.106
[Indexed for MEDLINE]

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