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Nucleic Acids Res. 1986 Nov 11;14(21):8605-13.

Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum.

Abstract

The nucleotide sequence of the celD gene, encoding the previously crystallized endoglucanase D of Clostridium thermocellum, is reported. The enzyme shares a conserved, reiterated domain with the COOH-terminal end of endoglucanases A and B from the same organism. The overexpression in Escherichia coli of celD subcloned in pUC8 appears to result from a translational fusion of the NH2-terminal end of the endoglucanase with the NH2-terminal end of beta-galactosidase.

PMID:
3024110
PMCID:
PMC311880
DOI:
10.1093/nar/14.21.8605
[Indexed for MEDLINE]
Free PMC Article

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