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Microsc Res Tech. 2018 Sep;81(9):1017-1023. doi: 10.1002/jemt.23067. Epub 2018 Sep 20.

The configuration of DMD and the maximum intensity projection method for improving contrast in DMD-based confocal microscope.

Author information

1
Shanghai Key Laboratory of Contemporary Optics System, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
2
Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania, 16802.

Abstract

In this article, an operation strategy of digital micromirror device (DMD) and the maximum intensity projection (MIP) image processing method are proposed to improve the contrast of images in confocal microscopy. First, the configuration of DMD is demonstrated and the effect of scanning unit size on image performance is analyzed. Then, the image processing method MIP is applied. According to the MIP method, only the maximum intensity projection point of the same pixel is chosen from every image, and the maximum intensity projection point exactly corresponds to the positon where mirror is at "on" position during the scanning process in DMD-based confocal microscope system,. Thus, high contrast of images can be achieved by using MIP. Finally, experiments are conducted to verify imaging performance by changing the parameter of scanning unit size and applying a MIP image processing technique. The results show that DMD scanning unit size and MIP image processing techniques play important roles in improving image contrast. Smaller scanning unit size of DMD improves axial contrast but greatly decreases the signal to noise ratio, which thus leads to reduced image contrast. Larger scanning unit size produces a better signal to noise ratio, thus better image contrast. However, a large S will sacrifice the processing time. Therefore, DMD scanning unit size should be smaller on the premise that image contrast can be satisfied. RESEARCH HIGHLIGHTS: Effect of DMD scanning unit size setting on image contrast is analyzed and verified. The maximum intensity projection (MIP) is investigated to improve the image contrast. Experiments are conducted to verify the enhancement of the image contrast.

KEYWORDS:

digital micromirror device (DMD); image contrast; scanning unit size; the maximum intensity projection image processing (MIP)

PMID:
30238548
DOI:
10.1002/jemt.23067

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