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Parasite. 2018;25:50. doi: 10.1051/parasite/2018045. Epub 2018 Sep 20.

Morphological and molecular characterization of Onchocerca fasciata (Nematoda, Onchocercidae) from dromedary camels (Camelus dromedarius) in Iran.

Author information

1
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Postal code: 7616914111 Kerman, Iran.
2
Genome Biology Division, New England Biolabs, Inc., 240 County Rd, Ipswich, MA 01938, USA.
3
Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, Str. prov. per Casamassima km 3, 70010 Valenzano, Bari, Italy.
4
Unité Molécules de Communication et Adaptation des Microorganismes, Muséum national d'Histoire naturelle, CNRS, 75231 Paris cedex 05, France.
5
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Postal code: 6517658978 Hamedan, Iran.

Abstract

Skin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata nodules (1.3-2.1 cm in diameter and 509-841 mg in weight) were detected on the neck region in 31.5% of dromedary camels examined in Kerman province, southeastern Iran. Of 38 isolated nodules, only 23 (60.5%) contained viable worms. Measurement and morphological analyses were performed on isolated female worms by light microscopy. The identification of O. fasciata specimens was confirmed by sequence analysis of two mitochondrial genes (12S rDNA and cox1), which showed 0.4% divergence from available O. fasciata sequences. In addition, a phylogeny of filarial nematodes was constructed, based on these two mitochondrial genes and five nuclear genes (18S rDNA, 28S rDNA, MyoHC, rbp1, hsp70); this indicated that O. fasciata belongs to clade ONC3 of Onchocercidae, with representatives of the genera Onchocerca and Dirofilaria. Within the genus Onchocerca, O. fasciata is grouped with bovine parasitic species and the human parasitic Onchocerca volvulus, which suggests an impact of domestication on the radiation of the genus. Data provided here on the distribution and morphology of O. fasciata contribute to the molecular identification and phylogenetic position of the species.

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