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Front Physiol. 2018 Sep 3;9:1219. doi: 10.3389/fphys.2018.01219. eCollection 2018.

Cell Type-Dependent Activation Sequence During Rhythmic Bursting in the PreBötzinger Complex in Respiratory Rhythmic Slices From Mice.

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Division of Physiome, Department of Physiology, Hyogo College of Medicine, Nishinomiya, Japan.
Department of Statistical Modeling, The Institute of Statistical Mathematics, Tachikawa, Japan.
Department of Statistical Science, School of Multidisciplinary Sciences, The Graduate University for Advanced Studies, Hayama, Japan.
Carl-Ludwig-Institute for Physiology, Faculty of Medicine, University of Leipzig, Leipzig, Germany.
Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany.
Clinic for Anesthesiology, University Medical Center Göttingen, Göttingen, Germany.
Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain, University Medical Center Göttingen, Göttingen, Germany.


Spontaneous respiratory rhythmic burst activity can be preserved in the preBötzinger Complex (preBötC) of rodent medullary transverse slices. It is known, that the activation sequence of inspiratory neurons in the preBötC stochastically varies from cycle to cycle. To test whether the activation timing of an inspiratory neuron depends on its neurotransmitter, we performed calcium imaging of preBötC neurons using double-transgenic mice expressing EGFP in GlyT2+ neurons and tdTomato in GAD65+ neurons. Five types of inspiratory neurons were identified using the fluorescence protein expression and the maximum cross-correlation coefficient between neuronal calcium fluctuation and field potential. Regarding the activation sequence, irregular type putative excitatory (GlyT2-/GAD65-) neurons and irregular type glycinergic (GlyT2+/GAD65-) neurons tended to be activated early, while regular type putative excitatory neurons, regular type glycinergic neurons tended to be activated later. In conclusion, the different cell types define a general framework for the stochastically changing activation sequence of inspiratory neurons in the preBötC.


activation sequence; activation timing; calcium imaging; inhibitory neuron; inspiratory neuron; preBötzinger complex; rhythmic burst

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