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Molecules. 2018 Sep 18;23(9). pii: E2386. doi: 10.3390/molecules23092386.

Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice.

Author information

1
Departamento de Nutrición Animal Dr. Fernando Pérez-Gil Romo, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico. claudia.delgadillop@incmnsz.mx.
2
Departamento de Nutrición Animal Dr. Fernando Pérez-Gil Romo, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico. mario.cuchilloh@incmnsz.mx.
3
Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), CDMX 04510, Mexico. arturono@unam.mx.
4
Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México (UNAM), CDMX 04510, Mexico. omarnoelmedina@gmail.com.
5
Instituto de Química, Universidad Nacional Autónoma de México (UNAM), CDMX 04510, Mexico. camanico2015@gmail.com.
6
Instituto de Química, Universidad Nacional Autónoma de México (UNAM), CDMX 04510, Mexico. mtrapan@yahoo.com.mx.
7
Colegio de Posgraduados en Ciencias Agrícolas, Puebla 72760, Mexico. zgerardo@colpos.mx.
8
Departamento de Nutrición Animal Dr. Fernando Pérez-Gil Romo, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico. maggiediazm@yahoo.com.
9
Departamento de Nutrición Aplicada y Educación Nutricional, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico. marselalejandra@yahoo.com.
10
Facultad de Química, Universidad Nacional Autónoma de México (UNAM), CDMX 04510, Mexico. yekann@hotmail.com.
11
Facultad de Química, Universidad Autónoma de Zacatecas, Zacatecas 98500, Mexico. vanelilla@gmail.com.
12
Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico. egomezinn@gmail.com.
13
Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico. ivan.inn@gmail.com.
14
Departamento de Inmunología y Reumatología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico. jfuruzawa@gmail.com.
15
Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Monterrey 64570, Mexico. maria.camachocn@uanl.edu.mx.
16
Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México (UNAM), CDMX 04510, Mexico. pedraza@unam.mx.

Abstract

BACKGROUND:

Acacia farnesiana (AF) pods have been traditionally used to treat dyspepsia, diarrhea and topically for dermal inflammation. Main objectives: (1) investigate the antioxidant activity and protection against oxidative-induced damage of six extracts from AF pods and (2) their capacity to curb the inflammation process as well as to down-regulate the pro-inflammatory mediators.

METHODS:

Five organic extracts (chloroformic, hexanic, ketonic, methanolic, methanolic:aqueous and one aqueous extract) were obtained and analyzed by UPLC-ESI-Q-oa/TOF-MS. Antioxidant activity (DPPH•, ORAC and FRAP assays) and lipid peroxidation (TBARS assay) were performed. Assessment of anti-inflammatory properties was made by the ear edema induced model in CD-1 mice and MPO activity assay. Likewise, histological analysis, IL-1β, IL-6, IL-10, TNF-α, COX measurements plus nitrite and immunohistochemistry analysis were carried out.

RESULTS:

Methyl gallate, gallic acid, galloyl glucose isomer 1, galloyl glucose isomer 2, galloyl glucose isomer 3, digalloyl glucose isomer 1, digalloyl glucose isomer 2, digalloyl glucose isomer 3, digalloyl glucose isomer 4, hydroxytyrosol acetate, quinic acid, and caffeoylmalic acid were identified. Both organic and aqueous extracts displayed antioxidant activity. All extracts exhibited a positive effect on the interleukins, COX and immunohistochemistry assays.

CONCLUSION:

All AF pod extracts can be effective as antioxidant and topical anti-inflammatory agents.

KEYWORDS:

Acacia farnesiana pods; antioxidant and anti-inflammatory activities; bioactive compounds; polyphenols

PMID:
30231503
PMCID:
PMC6225385
DOI:
10.3390/molecules23092386
[Indexed for MEDLINE]
Free PMC Article

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