Internalization of the pseudorabies virus via macropinocytosis analyzed by quantum dot-based single-virus tracking

Cheng Lv; Yi Lin; En-Ze Sun; Bo Tang; Jian Ao; Jia-Jia Wang; Zhi-Ling Zhang; Zhenhua Zheng; Hanzhong Wang; Dai-Wen Pang

doi:10.1039/c8cc05614e

Internalization of the pseudorabies virus via macropinocytosis analyzed by quantum dot-based single-virus tracking

Chem Commun (Camb). 2018 Oct 2;54(79):11184-11187. doi: 10.1039/c8cc05614e.

Authors

Cheng Lv¹, Yi Lin, En-Ze Sun, Bo Tang, Jian Ao, Jia-Jia Wang, Zhi-Ling Zhang, Zhenhua Zheng, Hanzhong Wang, Dai-Wen Pang

Affiliation

¹ Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, People's Republic of China. dwpang@whu.edu.cn.

PMID: 30229242
DOI: 10.1039/c8cc05614e

Abstract

With the aid of the single-virus tracking technique and the quantum dot-labeling strategy, the internalization of the pseudorabies virus (PrV) has been visualized in real time. The results demonstrate that macropinocytosis can be considered as a major pathway for PrV entering HeLa cells, which facilitates the development of therapeutics for virus-triggered diseases.

MeSH terms

Animals
HeLa Cells / virology
Herpesvirus 1, Suid / physiology*
Humans
Microscopy, Confocal / methods
Phosphatidylinositol 3-Kinase / metabolism
Pinocytosis*
Protein Kinase C / metabolism
Quantum Dots / chemistry*
Sodium-Hydrogen Exchangers / metabolism
Sorting Nexins / metabolism
Swine
Virus Internalization*
cdc42 GTP-Binding Protein / metabolism
p21-Activated Kinases / metabolism
rac1 GTP-Binding Protein / metabolism

Substances

RAC1 protein, human
SNX5 protein, human
Sodium-Hydrogen Exchangers
Sorting Nexins
Phosphatidylinositol 3-Kinase
PAK1 protein, human
p21-Activated Kinases
Protein Kinase C
cdc42 GTP-Binding Protein
rac1 GTP-Binding Protein