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Int J Biol Macromol. 2019 Feb 1;122:1035-1045. doi: 10.1016/j.ijbiomac.2018.09.050. Epub 2018 Sep 15.

Polysaccharides from tubers of Bletilla striata: Physicochemical characterization, formulation of buccoadhesive wafers and preliminary study on treating oral ulcer.

Author information

1
Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
2
College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, PR China.
3
Department of Chemistry, Stony Brook University, Stony Brook, NY 11794, United States.
4
Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China. Electronic address: quyan028@126.com.

Abstract

The purpose of this research is to arouse people's cognition of the dual role of Bletilla striata polysaccharide (BSP)-a kind of biocompatible, biodegradable natural biological materials with myriad pharmacological activities. BSP was extracted and characterized firstly. The results indicated that BSP was composed of mannose and glucose. The backbone of BSP mainly consisted of (1 → 4)-linked β-D-mannopyranose. Afterwards, A 32 factorial design was employed for optimization of lyophilized wafers for the treatment of traumatic oral ulcer with concentrations of BSP and glycerin as independent variables. Tensile strength, mucoadhesive strength, and swelling index were measured as response variables. It was determined that the wafer prepared from a solution containing 4% (w/v) BSP and 7% (w/v) glycerin was the optimal formulation. The wafers exhibited sufficient mechanical strength and suitable surface pH. Morphology of the wafers was observed. DSC/TGA thermograms showed the presence of three peaks weight loss events and decomposition patterns. Moreover, in vivo examinations indicated that the BSP wafer significantly promoted ulcer healing at 7 days, while no statistically significant difference was observed on day 12. In conclusion, this study suggests that BSP is an ideal biomaterial for mucosal adhesion agents, playing a nontrivial role in promoting oral ulcer healing.

KEYWORDS:

3(2) factorial design; Bletilla striata polysaccharide; Lyophilized wafer; Physicochemical characterization; Wound healing

PMID:
30227203
DOI:
10.1016/j.ijbiomac.2018.09.050
[Indexed for MEDLINE]

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