Inhibition of epithelial‑mesenchymal transition in gastric cancer cells by miR‑711‑mediated downregulation of CD44 expression

Oncol Rep. 2018 Nov;40(5):2844-2853. doi: 10.3892/or.2018.6681. Epub 2018 Sep 4.

Abstract

Gastric cancer is a common malignancy worldwide. The prognosis of early stage gastric cancer patients has significantly improved in recent years. However, in progressive stage gastric cancer patients, the prognosis remains relatively poor due to tumor metastases. In our previous study, we showed that the expression of miR‑711 in gastric cancer tissues is low, and restoration of miR‑711 inhibited the invasion and migration and the occurrence of epithelial‑mesenchymal transition (EMT) in gastric cancer cells. Yet, the mechanisms involved in these processes remain unknown. In the present study, we demonstrated that miR‑711‑mediated downregulation of CD44 expression inhibited EMT of gastric cancer cells in vitro and in vivo by downregulating vimentin protein expression and upregulating E‑cadherin protein expression through transfection, qRT‑PCR and western blotting. Therefore, miR‑711 may provide a promising target for EMT‑related therapy for gastric cancer.

MeSH terms

  • Animals
  • Cadherins / genetics*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Epithelial-Mesenchymal Transition / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Hyaluronan Receptors / genetics*
  • Mice
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics
  • Neoplasm Staging
  • Prognosis
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / therapy
  • Transfection
  • Xenograft Model Antitumor Assays

Substances

  • CD44 protein, human
  • Cadherins
  • Hyaluronan Receptors
  • MIRN711 microRNA, human
  • MicroRNAs