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Nat Methods. 2018 Nov;15(11):917-920. doi: 10.1038/s41592-018-0111-2. Epub 2018 Sep 17.

Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy.

Author information

1
Allen Institute for Cell Science, Seattle, WA, USA.
2
Allen Institute for Brain Science, Seattle, WA, USA.
3
Allen Institute for Cell Science, Seattle, WA, USA. gregj@alleninstitute.org.

Abstract

Understanding cells as integrated systems is central to modern biology. Although fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, is slow, and can damage cells. We present a label-free method for predicting three-dimensional fluorescence directly from transmitted-light images and demonstrate that it can be used to generate multi-structure, integrated images. The method can also predict immunofluorescence (IF) from electron micrograph (EM) inputs, extending the potential applications.

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PMID:
30224672
PMCID:
PMC6212323
DOI:
10.1038/s41592-018-0111-2
[Indexed for MEDLINE]
Free PMC Article

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