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Anal Chim Acta. 2018 Dec 4;1035:168-174. doi: 10.1016/j.aca.2018.06.039. Epub 2018 Jun 15.

A novel and sensitive chemiluminescence immunoassay based on AuNCs@pepsin@luminol for simultaneous detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether.

Author information

1
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: zhangzhen@ujs.edu.cn.
2
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
3
School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
4
CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Biomass Energy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
5
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: menghui@ujs.edu.cn.

Abstract

A novel chemiluminescence immunoassay based on luminol-modified gold nanoclusters (AuNCs@Peps@luminol) was developed for simultaneous detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE) and tetrabromobisphenol A mono(hydroxyethyl) ether (TBBPA-MHEE), an important derivative and byproduct of tetrabromobisphenol A (TBBPA), respectively. In the system, alkaline phosphatase (ALP) was labeled on the second antibody (Ab2) for signal amplification. When ALP-Ab2 was captured by antigen-primary antibody (Ab1) complex, disodium phenyl phosphate (PPNa) generated massive phenol under the catalysis of ALP, markedly inhibiting the chemiluminescence intensity of AuNCs@Peps@luminol. Under the optimized conditions, the calculated detection of limit (LOD, 90% inhibition) was 0.078 μg/L for TBBPA-DHEE with a linear range of 0.23-9.32 μg/L, which was lower 9 times than that of conventional ELISA with the same antibody. In addition, our method showed satisfactory accuracy and precision (recoveries, 88.00-113.4%; CV, 2.75-8.14%), it can be applied to systematically investigate the concentration of the trace TBBPA-DHEE and TBBPA-MHEE in environmental and food samples.

KEYWORDS:

Antibody; AuNCs@pepsin@luminol; Derivatives; Immunoassay; TBBPA

PMID:
30224136
DOI:
10.1016/j.aca.2018.06.039
[Indexed for MEDLINE]

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