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Cell Biochem Biophys. 2019 Mar;77(1):99-107. doi: 10.1007/s12013-018-0857-2. Epub 2018 Sep 14.

Superoxide Formation in Cardiac Mitochondria and Effect of Phenolic Antioxidants.

Author information

1
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
2
National Medical Research Centre for Cardiology, Moscow, Russia.
3
Bach Institute of Biochemistry, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
4
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia. ruuge@physics.msu.ru.
5
National Medical Research Centre for Cardiology, Moscow, Russia. ruuge@physics.msu.ru.

Abstract

Since mitochondria are the main cellular source of reactive oxygen species, it is important to study the effect of dietary phenolic compounds on the level of ROS in these organelles. Using the EPR spectroscopy and TIRON probe, the ability of the investigated phenols (quercetin, rutin, caffeic acid, curcumin, and resveratrol) to scavenge superoxide anion radicals generated by isolated heart mitochondria of Wistar rats under variable oxygen partial pressure was studied. It was shown that during a 10 min incubation, caffeic acid in concentrations of 10-500 μM most effectively scavenged superoxide radicals formed in the complex III of the mitochondrial respiratory chain. A comparable antioxidant effect of rutin under these experimental conditions was observed at higher concentrations of 1-10 mM. The antioxidant activity of quercetin in the concentration range of 10-500 μM during the first minutes of incubation was higher than that of caffeic acid. Of the phenolic compounds studied, curcumin had the least effect on the superoxide radicals.

KEYWORDS:

Antioxidants; EPR spectroscopy; Heart mitochondria; Hypoxia; Phenolic compounds; Reactive oxygen species

PMID:
30218405
DOI:
10.1007/s12013-018-0857-2
[Indexed for MEDLINE]

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