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Exp Ther Med. 2018 Oct;16(4):2867-2874. doi: 10.3892/etm.2018.6566. Epub 2018 Aug 2.

The function of MMP-28/TGF-β induced cell apoptosis in human glioma cells.

Author information

1
Department of Neurosurgery, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China.
2
Department of Neurology, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China.
3
Department of Production, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China.
4
Department of CT, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China.
5
Department of Cycle of Internal, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China.

Abstract

The aim of the present study was to assess the expression status of matrix metalloproteinase (MMP)-28 and to investigate its molecular mechanisms in glioma cells. MicroRNA (miRNA) reverse transcription-quantitative polymerase chain reaction was used to analyze the expression of MMP-28 and transforming growth factor (TGF)-β expression in glioma patients and healthy volunteers. MTT and Transwell assays were conducted to determine cell growth and metastasis, respectively. Annexin V/propidium iodide staining was also employed to measure cell apoptosis. MMP-28 and TGF-β protein expression were measured using western Blot analysis. The results indicated that MMP-28 and TGF-β expression was downregulated in glioma patients, when compared with the normal group. Overall survival and disease-free survival of patients with a low expression of MMP-28 were lower than those with high MMP-28 expression. Overexpression of MMP-28 induced TGF-β protein expression, while downregulation of MMP-28 suppressed TGF-β protein expression in glioma cell. The downregulation of MMP-28 reduced the cell growth and apoptosis of glioma cell via the suppression of TGF-β. By contrast, upregulation of MMP-28 induced cell growth and reduced the apoptosis of glioma cells by activating TGF-β. In addition, the TGF-β inhibitor attenuated the effects of MMP-28 in glioma cells. Collectively, the results indicated that MMP-28 was able to induce TGF-β in human glioma cells.

KEYWORDS:

glioma cell; matrix metalloproteinase-28; transforming growth factor-β

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