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Onco Targets Ther. 2018 Aug 28;11:5239-5252. doi: 10.2147/OTT.S171539. eCollection 2018.

miR-361-5p inhibits glioma migration and invasion by targeting SND1.

Liu J1,2, Yang J3, Yu L3, Rao C2,4,5, Wang Q2,4,5, Sun C2,4,5, Shi C2,4,5, Hua D2,4,5, Zhou X2,4,5, Luo W2,4,5, Wang R2,4,5, Li W1, Yu S2,4,5.

Author information

Department of Neurosurgery and Shenzhen Key Laboratory of Neurosurgery, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen University School of Medicine, Shenzhen 518035, People's Republic of China,
Department of Neuropathology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China,
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences of Tianjin Medical University, Tianjin 300070, People's Republic of China.
Department of Neuropathology, Key Laboratory of Post-Trauma Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin 300052, People's Republic of China,
Department of Neuropathology, Tianjin Key Laboratory of Injuries, Variations and Regeneration of the Nervous System, Tianjin 300052, People's Republic of China,



Downregulation of miR-361-5p contributes to epithelial-mesenchymal transition of glioma cells. However, the relevance of miR-361-5p to migration and invasion of gliomas remains unknown.

Materials and methods:

The relationship between miR-361-5p and SND1 expression was analyzed in 120 human gliomas and 8 glioma cell lines by in situ hybridization, immunohistochemistry, and Western blot. Dual-luciferase reporter assay was used to identify SND1 as a target of miR-361-5p. The mechanisms through which miR-361-5p inhibits glioma cell migration and invasion were studied by in vitro assays.


miR-361-5p expression was significantly downregulated in glioma tissues and glioma cell lines, and was inversely correlated with glioma grades. However, SND1 expression was positively correlated with glioma grades and inversely correlated with miR-361-5p expression. miR-361-5p overexpression suppressed glioma cell migration and invasion through targeting SND1 and subsequently decreasing MMP-2 expression. In glioma cell lines, SND1 overexpression could partly reverse the antitumor effects of miR-361-5p.


The findings provide evidence that miR-361-5p directly targets SND1 to degradation and then reduces MMP-2 gene transcription, thus inhibiting glioma migration and invasion. miR-361-5p is an important tumor suppressor and a novel diagnostic biomarker of glioma, and miR-361-5p and SND1 are potential therapeutic candidates for malignant gliomas.


SND1; glioma; invasion; miR-361-5p; migration

Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

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