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Cell Rep. 2018 Sep 11;24(11):3072-3086. doi: 10.1016/j.celrep.2018.08.013.

Decoding the Regulatory Logic of the Drosophila Male Stem Cell System.

Author information

1
Centre for Organismal Studies (COS) Heidelberg, Heidelberg University, 69120 Heidelberg, Germany.
2
Centre for Organismal Studies (COS) Heidelberg, Heidelberg University, 69120 Heidelberg, Germany; Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
3
Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany; Department of Cell and Molecular Biology, University of Heidelberg, 69120 Heidelberg, Germany.
4
Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany.
5
Institut Jacques Monod, University Paris-Diderot, 75205 Paris, France.
6
Centre for Organismal Studies (COS) Heidelberg, Heidelberg University, 69120 Heidelberg, Germany. Electronic address: ingrid.lohmann@cos.uni-heidelberg.de.

Abstract

The niche critically controls stem cell behavior, but its regulatory input at the whole-genome level is poorly understood. We elucidated transcriptional programs of the somatic and germline lineages in the Drosophila testis and genome-wide binding profiles of Zfh-1 and Abd-A expressed in somatic support cells and crucial for fate acquisition of both cell lineages. We identified key roles of nucleoporins and V-ATPase proton pumps and demonstrate their importance in controlling germline development from the support side. To make our dataset publicly available, we generated an interactive analysis tool, which uncovered conserved core genes of adult stem cells across species boundaries. We tested the functional relevance of these genes in the Drosophila testis and intestine and found a high frequency of stem cell defects. In summary, our dataset and interactive platform represent versatile tools for identifying gene networks active in diverse stem cell types.

KEYWORDS:

DamID; Drosophila testis; V-ATPases; abd-A; homeodomain transcription factors; nucleoporins; somatic support cells; stem cells; targeted DamID; zfh-1

PMID:
30208329
DOI:
10.1016/j.celrep.2018.08.013
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