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Curr Pharm Des. 2018 Sep 11. doi: 10.2174/1381612824666180911120039. [Epub ahead of print]

Modified Ldl Particles Activate Inflammatory Pathways In Monocyte-Derived Macrophages: Transcriptome Analysis.

Author information

1
Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 125315 Moscow. Russian Federation.
2
Department of Cellular and Molecular Medicine, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 1138510. Japan.
3
Laboratory of postgenomic research, Federal Research and Clinical Center of Physical-Chemical Medicine, 119435 Moscow. Russian Federation.
4
GW School of Medicine and Health Sciences, George Washington University, Washington, DC 20037. Russian Federation.
5
Laboratory of Medical Genetics, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, 121552 Moscow. Russian Federation.
6
Biosoft.ru Ltd, 630001 Novosibirsk. Russian Federation.
7
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991. Russian Federation.
8
Kalen Biomedical, LLC, Montgomery Village, MD 20886. United States.
9
Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892. United States.
10
GW School of Medicine and Health Sciences, George Washington University, Washington, DC 20037. United States.

Abstract

A hallmark of atherosclerosis is its complex pathogenesis, which is dependent on altered cholesterol metabolism and inflammation. Both arms of pathogenesis involve myeloid cells. Monocytes migrating into the arterial walls interact with modified low-density lipoprotein (LDL) particles, accumulate cholesterol and convert into foam cells, which promote plaque formation and also contribute to inflammation by producing pro-inflammatory cytokines. A number of studies characterized transcriptomics of macrophages following interaction with modified LDL, and revealed alteration of the expression of genes responsible for inflammatory response and cholesterol metabolism. However, it is still unclear how these two processes are related to each other to contribute to atherosclerotic lesion formation. We attempted to identify the main mater regulator genes in macrophages treated with atherogenic modified LDL using a bioinformatics approach. We found that most of the identified genes were involved in inflammation, and none of them was implicated in cholesterol metabolism. Among the key identified genes were interleukin (IL)-7, IL-7 receptor, IL-15 and CXCL8. Our results indicate that activation of the inflammatory pathway is the primary response of the immune cells to modified LDL, while the lipid metabolism genes may be a secondary response triggered by inflammatory signalling.

KEYWORDS:

LDL; atherosclerosis; inflammation; macrophages; modified LDL; pro-inflammatory signaling

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