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Methods Mol Biol. 2018;1843:107-113. doi: 10.1007/978-1-4939-8704-7_9.

Western Blotting and Co-immunoprecipitation of Endogenous STIM/ORAI and Protein Partners.

Author information

1
INSERM U1003, Laboratoire d'Excellence, Canaux Ioniques d'Intérêt Thérapeutique, Équipe Labellisée Par la Ligue Nationale Contre le Cancer, SIRIC ONCOLille, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
2
INSERM U1003, Laboratoire d'Excellence, Canaux Ioniques d'Intérêt Thérapeutique, Équipe Labellisée Par la Ligue Nationale Contre le Cancer, SIRIC ONCOLille, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France. natacha.prevarskaya@univ-lille1.fr.

Abstract

The characterization of protein-protein interactions through methods such as co-immunoprecipitation, followed by Western blot analysis, is a crucial step in the understanding of protein functions and the biology of the cell. Since the discovery of ORAI and STIM proteins as component of store-operated channel (SOC), overexpressing systems have been used to demonstrate how ORAI and STIM can associate with physiological and pathological conditions. Here we describe a protocol allowing endogenous studies.

KEYWORDS:

Co-immunoprecipitation; Endogenous; ORAI; STIM; Western blot

PMID:
30203281
DOI:
10.1007/978-1-4939-8704-7_9
[Indexed for MEDLINE]

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