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RETRACTED ARTICLE

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Sci Rep. 2018 Sep 10;8(1):13562. doi: 10.1038/s41598-018-31971-9.

Ultra-diluted Toxicodendron pubescens attenuates pro-inflammatory cytokines and ROS- mediated neuropathic pain in rats.

Author information

1
Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur- 425405, Dist. Dhule, Maharashtra, India.
2
School of Biotechnology, Kalinga Institute of Industrial technology (a deemed to be University), Campus-11, Patia, Bhubaneswar, Odisha, Pin-751024, India.
3
SVKM's Institute of Pharmacy, Dhule-424001, Dist-Dhule, Maharashtra, India.
4
Janmangal Homeopathy and Wellness Centre, Bopal, Ahmedabad, Gujarat, 380058, India.
5
Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur- 425405, Dist. Dhule, Maharashtra, India. xplore.remedies@gmail.com.
6
Department of Pharmacology & Therapeutics, College of Medicine & Health Sciences, UAE University, Al Ain, UAE. shreeshojha@uaeu.ac.ae.
7
School of Biotechnology, Kalinga Institute of Industrial technology (a deemed to be University), Campus-11, Patia, Bhubaneswar, Odisha, Pin-751024, India. cnkundu@kiitbiotech.ac.in.

Abstract

Despite the availability of multiple therapeutic agents, the search for novel pain management of neuropathic pain is still a challenge. Oxidative stress and inflammatory signaling are prominently involved in clinical manifestation of neuropathic pain. Toxicodendron pubescens, popularly known as Rhus Tox (RT) is recommended in alternative medicines as an anti-inflammatory and analgesic remedy. Earlier, we reported anti-inflammatory, anti-arthritic and immunomodulatory activities of Rhus Tox. In continuation, we evaluated antinociceptive efficacy of Rhus Tox in the neuropathic pain and delineated its underlying mechanism. Initially, in-vitro assay using LPS-mediated ROS-induced U-87 glioblastoma cells was performed to study the effect of Rhus Tox on reactive oxygen species (ROS), anti-oxidant status and cytokine profile. Rhus Tox decreased oxidative stress and cytokine release with restoration of anti-oxidant systems. Chronic treatment with Rhus Tox ultra dilutions for 14 days ameliorated neuropathic pain revealed as inhibition of cold, warm and mechanical allodynia along with improved motor nerve conduction velocity (MNCV) in constricted nerve. Rhus Tox decreased the oxidative and nitrosative stress by reducing malondialdehyde (MDA) and nitric oxide (NO) content, respectively along with up regulated glutathione (GSH), superoxide dismutase (SOD) and catalase activity in sciatic nerve of rats. Notably, Rhus Tox treatment caused significant reductions in the levels of tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) as compared with CCI-control group. Protective effect of Rhus Tox against CCI-induced sciatic nerve injury in histopathology study was exhibited through maintenance of normal nerve architecture and inhibition of inflammatory changes. Overall, neuroprotective effect of Rhus Tox in CCI-induced neuropathic pain suggests the involvement of anti-oxidative and anti-inflammatory mechanisms.

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