Format

Send to

Choose Destination
Sci Rep. 2018 Sep 10;8(1):13509. doi: 10.1038/s41598-018-31998-y.

New Optical Imaging Reporter-labeled Anaplastic Thyroid Cancer-Derived Extracellular Vesicles as a Platform for In Vivo Tumor Targeting in a Mouse Model.

Gangadaran P1,2, Li XJ1,2,3, Kalimuthu SK1,2, Min OJ1,2, Hong CM1,2, Rajendran RL1,2, Lee HW1,2, Zhu L1,2, Baek SH1,2, Jeong SY1,2, Lee SW1,2, Lee J1,2, Ahn BC4,5.

Author information

1
Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
2
Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
3
Department of Radiology, Taian City Central Hospital, Taian, People's Republic of China.
4
Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea. abc2000@knu.ac.kr.
5
Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea. abc2000@knu.ac.kr.

Abstract

Extracellular vesicles (EVs), originating from multivesicular bodies by invagination of the endosomal membrane, are communication channels between distant cells. They are natural carriers of exogeneous cellular materials and have been exploited as drug delivery carriers in various diseases. Here, we found that tumor cell-derived EVs can be used as efficient targets in tumors by monitoring with an optical reporter system. Anaplastic thyroid cancer (CAL62) cell-derived EVs with Renilla luciferase (Rluc) were used to target CAL62 tumors in a mouse model. Optical imaging revealed that cancer cell-derived EVs (EV-CAL62/Rluc) targeted the original tumor (CAL62) in mice within 30 min after systemic injection. Furthermore, fluorescence imaging revealed that EV-CAL62/Rluc were internalized into CAL62 tumors in the mice. Ex vivo Optical imaging further confirmed the in vivo finding. Here, we successfully monitored the tumor targeting ability of tumor cell-derived EVs by optical imaging. Based on these results, tumor cell-derived EVs are highly effective natural carriers for drug delivery for cancer therapies.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center