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Molecules. 2018 Aug 30;23(9). pii: E2190. doi: 10.3390/molecules23092190.

Biofilm Inhibitory Abscisic Acid Derivatives from the Plant-Associated Dothideomycete Fungus, Roussoella sp.

Author information

1
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. chayanard91@gmail.com.
2
Department of Microbial Drugs, Helmholtz Centre for Infection Research and German Centre for Infection Research (DZIF), partner site Hannover/Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany. agmacabeo@ust.edu.ph.
3
Laboratory for Organic Reactivity, Discovery and Synthesis (LORDS), Research Center for the Natural and Applied Sciences, University of Santo Tomas, 1015 Manila, Philippines. agmacabeo@ust.edu.ph.
4
Department of Microbial Drugs, Helmholtz Centre for Infection Research and German Centre for Infection Research (DZIF), partner site Hannover/Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany. kamilatomoko@gmail.com.
5
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. kdhyde3@gmail.com.
6
Department of Microbial Drugs, Helmholtz Centre for Infection Research and German Centre for Infection Research (DZIF), partner site Hannover/Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany. Marc.Stadler@helmholtz-hzi.de.

Abstract

Roussoella species are well recorded from both monocotyledons and dicotyledons. As part of a research program to discover biologically active compounds from plant-associated Dothideomycetes in Thailand, the strain Roussoella sp. (MFLUCC 17-2059), which represents an undescribed species, was isolated from Clematis subumbellata Kurz, fermented in yeast-malt medium and explored for its secondary metabolite production. Bioassay-guided fractionation of the crude extract yielded the new abscisic acid derivative, roussoellenic acid (1), along with pestabacillin B (2), a related congener, and the cyclodipeptide, cyclo(S-Pro-S-Ile) (3). The structure of 1 was determined by 2D NMR spectroscopy and HR-ESIMS data analysis. Compounds 1 and 2 showed inhibitory activity on biofilm formation by Staphylococcus aureus. The biofilm formation of S. aureus was reduced to 34% at 16 µg/mL by roussoellenic acid (1), while pestabacillin B (2) only showed 36% inhibition at 256 µg/mL. In addition, compound 1 also had weak cytotoxic effects on L929 murine fibroblasts and human KB3-1 cancer cells.

KEYWORDS:

Anti-biofilm; Ascomycota; Pleosporales; structure elucidation

PMID:
30200229
PMCID:
PMC6225182
DOI:
10.3390/molecules23092190
[Indexed for MEDLINE]
Free PMC Article

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