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Thromb Res. 2018 Oct;170:156-164. doi: 10.1016/j.thromres.2018.08.024. Epub 2018 Sep 1.

Alterations in the parameters of classic, global, and innovative assays of hemostasis caused by sample transportation via pneumatic tube system.

Author information

1
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Samory Mashela str., 1, Moscow, Russia.
2
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Samory Mashela str., 1, Moscow, Russia; Center for Theoretical Problems of Physicochemical Pharmacology, 119991 Kosygina str., 4, Moscow, Russia. Electronic address: ekaterina_koltsova@bk.ru.
3
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Samory Mashela str., 1, Moscow, Russia; Center for Theoretical Problems of Physicochemical Pharmacology, 119991 Kosygina str., 4, Moscow, Russia.
4
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Samory Mashela str., 1, Moscow, Russia; Center for Theoretical Problems of Physicochemical Pharmacology, 119991 Kosygina str., 4, Moscow, Russia; M.V. Lomonosov Moscow State University, 119991 Leninskie Gory, 1 build. 2, Moscow, Russia; Moscow Institute of Physics and Technology, 141701 Institutsky lane, 9, Dolgoprudny, Russia.

Abstract

BACKGROUND:

Pneumatic tube system (PTS) is an integral part of large medical facilities providing rapid interconnection between units within the hospital and often used to transport blood samples. The aim of our study was to compare a wide variety of hemostasis assays to identify assays sensitive to this transport method and diagnostic relevance of the alterations.

METHODS:

Routine coagulation and platelet tests (APTT, PT, TT, fibrinogen, light transmission aggregometry (LTA) with ADP, collagen, ristomycin and epinephrine), whole blood flow cytometry platelet function test (levels of CD42b, CD61, CD62P, PAC1, annexin V binding and mepacrine release) and global coagulation tests (thromboelastography (TEG), thrombin generation (TGT), thrombodynamics (TD), thrombodynamics-4D (TD-4D)) were determined in PTS- and manually transported samples of 10 healthy volunteers.

RESULTS:

There were no significant differences between the values of APTT, PT, TT or fibrinogen between the samples transported by PTS or manually. The results for LTA demonstrated increase in the collagen-induced aggregation (84 ± 7% versus 73 ± 5%), while the response to epinephrine was decreased (58 ± 20% versus 72 ± 7.4%). Flow cytometry-based platelet function test showed a pre-activation of platelets by PTS-transportation while all integral assays of coagulation tested in the present study (TEG, TGT, TD, TD-4D) demonstrated a hypercoagulation shift.

CONCLUSIONS:

Transportation by PTS caused significant shifts in parameters of functional and integral assays that exceeded parameter variation values and sometimes even were comparable to normal ranges. The results obtained in this study indicate that using of PTS for such assays may cause sufficient alterations of results and can lead to patient's mistreatment.

KEYWORDS:

Assay variability; Flow cytometry; Integral assays of hemostasis; Platelet functional activity; Pneumatic tube transport; Thrombodynamics

PMID:
30196193
DOI:
10.1016/j.thromres.2018.08.024
[Indexed for MEDLINE]

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