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Biosens Bioelectron. 2019 Jan 1;123:223-229. doi: 10.1016/j.bios.2018.08.064. Epub 2018 Aug 28.

Development of a rapid and sensitive electrochemical biosensor for detection of human norovirus via novel specific binding peptides.

Author information

1
Department of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Halal Industrialization Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
2
Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, Ansung 17546, Republic of Korea.
3
Department of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Halal Industrialization Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea; Department of Applied Chemistry, S. V. National Institute of Technology, Surat 395007, Gujarat, India.
4
Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Republic of Korea. Electronic address: tjpark@cau.ac.kr.
5
Department of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Halal Industrialization Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea. Electronic address: jppark@dhu.ac.kr.

Abstract

Human noroviruses cause acute foodborne gastroenteritis outbreaks worldwide. In this study, a highly sensitive and selective electrochemical biosensor was fabricated for the detection of human norovirus using novel peptides as recognition elements. The electrochemical biosensor was fabricated by assembling of eight novel peptides separately on the gold electrode and investigated their efficiencies for sensing human noroviruses. Among eight peptides, NoroBP peptide coated onto the gold electrode exhibited a high binding affinity towards human noroviruses, resulting a progressive decrease in current signals with increasing concentration of human norovirus (0-105 copies/mL). As a result, NoroBP-nonFoul(FlexL)2-coated gold electrode acts an efficient electrochemical biosensor for highly selective detection of human norovirus with a detection limit of 1.7 copies/mL, which is 3-fold lower than the reported methods. The developed electrochemical biosensor was successfully applied to detect human norovirus prepared by standard procedure from oyster, which suggests that the developed biosensor can be used as a very sensitive and selective point-of-care bioanalytical platform for the detection of human norovirus in various food samples.

KEYWORDS:

Biosensor; Electrochemical impedance spectroscopy; Human norovirus; Specific binding peptides

PMID:
30195404
DOI:
10.1016/j.bios.2018.08.064
[Indexed for MEDLINE]

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