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Sci Rep. 2018 Sep 7;8(1):13437. doi: 10.1038/s41598-018-31542-y.

Neuronal microRNA regulation in Experimental Autoimmune Encephalomyelitis.

Author information

1
McGill University, Montréal Neurological Institute, Montréal, QC, H3A 2B4, Canada.
2
Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
3
McGill University, Goodman Cancer Research Centre, Montréal, H3A 1A3, Canada.
4
McGill University, Departments of Microbiology & Immunology and Biochemistry, Montréal, QC, H3G 0B1, Canada.
5
Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
6
McGill University, Montréal Neurological Institute, Montréal, QC, H3A 2B4, Canada. alyson.fournier@mgill.ca.

Abstract

Multiple sclerosis (MS) is an autoimmune, neurodegenerative disease but the molecular mechanisms underlying neurodegenerative aspects of the disease are poorly understood. microRNAs (miRNAs) are powerful regulators of gene expression that regulate numerous mRNAs simultaneously and can thus regulate programs of gene expression. Here, we describe miRNA expression in neurons captured from mice subjected to experimental autoimmune encephalomyelitis (EAE), a model of central nervous system (CNS) inflammation. Lumbar motor neurons and retinal neurons were laser captured from EAE mice and miRNA expression was assessed by next-generation sequencing and validated by qPCR. We describe 14 miRNAs that are differentially regulated in both neuronal subtypes and determine putative mRNA targets though in silico analysis. Several upregulated neuronal miRNAs are predicted to target pathways that could mediate repair and regeneration during EAE. This work identifies miRNAs that are affected by inflammation and suggests novel candidates that may be targeted to improve neuroprotection in the context of pathological inflammation.

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