Format

Send to

Choose Destination
Sci Rep. 2018 Sep 7;8(1):13455. doi: 10.1038/s41598-018-31640-x.

Cell type-resolved human lung lipidome reveals cellular cooperation in lung function.

Author information

1
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
2
Department of Pediatrics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY, 14642, USA.
3
Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 45229, USA.
4
Genomics Research Center, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY, 14642, USA.
5
Flow Cytometry Core Facility, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY, 14642, USA.
6
Department of Pathology, Seattle Children's Hospital, Seattle, WA, 98105, USA.
7
Texas Advanced Computing Center, University of Texas at Austin, Austin, TX, 78712, USA.
8
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA. charles.ansong@pnnl.gov.

Abstract

Cell type-resolved proteome analyses of the brain, heart and liver have been reported, however a similar effort on the lipidome is currently lacking. Here we applied liquid chromatography-tandem mass spectrometry to characterize the lipidome of major lung cell types isolated from human donors, representing the first lipidome map of any organ. We coupled this with cell type-resolved proteomics of the same samples (available at Lungmap.net). Complementary proteomics analyses substantiated the functional identity of the isolated cells. Lipidomics analyses showed significant variations in the lipidome across major human lung cell types, with differences most evident at the subclass and intra-subclass (i.e. total carbon length of the fatty acid chains) level. Further, lipidomic signatures revealed an overarching posture of high cellular cooperation within the human lung to support critical functions. Our complementary cell type-resolved lipid and protein datasets serve as a rich resource for analyses of human lung function.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center